Dna Supercoiling and Bacterial Gene Expression
نویسندگان
چکیده
منابع مشابه
Transcription-driven DNA supercoiling and gene expression control.
DNA supercoiling plays important roles in gene expression regulation, although, the underlying mechanisms whereby DNA supercoiling modulates gene expression remain elusive. The fact that the transcription process itself generates DNA supercoiling has further complicated the issue. Transcription-driven DNA supercoiling is local and transient. Such a DNA supercoiling effect is likely to play impo...
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The intracellular pathogen Chlamydia has an unusual developmental cycle marked by temporal expression patterns whose mechanisms of regulation are largely unknown. To examine if DNA topology can regulate chlamydial gene expression, we tested the in vitro activity of five chlamydial promoters at different superhelical densities. We demonstrated for the first time that individual chlamydial promot...
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Type II DNA topoisomerases permit passages of double stranded DNA segments through each other and this is achieved via a complex mechanism involving a transient cleavage of one duplex, a passage of the second duplex through the topoisomerase-spanned cleavage site and finally resealing of the cut duplex. Type II DNA topoisomerases facilitate many DNA transactions requiring manipulation of long D...
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We show that several interacting environmental factors influence the topology of intracellular DNA. Negative supercoiling of DNA in vivo is increased by anaerobic growth and is also influenced by growth phase. The tonB promoter of Escherichia coli and Salmonella typhimurium was found to be highly sensitive to changes in DNA supercoiling. Expression was increased by novobiocin, an inhibitor of D...
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Despite the fact that in living cells DNA molecules are long and highly crowded, they are rarely knotted. DNA knotting interferes with the normal functioning of the DNA and, therefore, molecular mechanisms evolved that maintain the knotting and catenation level below that which would be achieved if the DNA segments could pass randomly through each other. Biochemical experiments with torsionally...
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ژورنال
عنوان ژورنال: Science Progress
سال: 2006
ISSN: 0036-8504,2047-7163
DOI: 10.3184/003685006783238317